Evaluation of the antibacterial activity of honey biology essay
Phenolic compounds have a significant effect on the antibacterial activity of honey against S. epidermidis and P. aeruginosa as a group rather than as an individual. The formation of and TPC are closely related to the antibacterial activity against S. epidermidis, demonstrating its importance in the action of honey against Gram-positive. 1 Introduction. Honey is a natural, sweet and homogeneous substance produced by honey bees from the nectar or excretions of plants or excretions of plant-sucking insects on the living parts of plants, which the bees collect and transform. It consists of a mixture of -85 sugars, 15-17 water, 0.1-0.4 proteins, and, to a lesser extent, honey possesses health-promoting properties. However, industrial processing and manipulation of raw honey can impair its biological activities, including its antibacterial activity. Therefore, this study aimed to compare honey's antibacterial activity, total protein content and abundance of the most dominant bee species. In general, the antibacterial effect of honey has been associated with the presence of MGO in MH and hydrogen peroxide in SH. Other factors that contribute to honey's antibacterial activities include low pH, osmotic pressure, bee defensin-1, an antibacterial peptide derived from bees, low protein content and hyperosmolality. The “direct” agar diffusion test is primarily used to screen potential antibiotic producers such as actinomycetes, 18, 19 through their direct exposure to an indicator strain, also called a test strain, on solid medium. First, the producer is spotted and incubated under optimal conditions for actinomycetes, 28-37 C, 5 - The antioxidant effect of honey has a direct and significant relationship with the plant and the geographical origin of honey, and the presence of some plant families increases significantly the. Therefore, the antibacterial activity of honey may vary depending on the geographical and botanical origin of the raw material 158 159 160, since the content of polyphenols can influence its composition. Honey is a natural remedy for various health problems. It exhibits a prebiotic effect on the intestinal microbiome, including lactobacilli, which is essential for maintaining intestinal health and regulating the immune system. In addition, monofloral honey may exhibit special therapeutic properties. We investigated the prebiotic properties of some legume honeys and 2. Phenolic content TPC We determined the total phenolic content using a modified Folin-Ciocalteu method as described by Beretta et al. 2005. From each honey sample ml of distilled water was treated, mixed and filtered using a qualitative filter. of this solution was mixed, l Folin-Ciocalteu, This review focuses on the phytochemical composition of manuka honey and on its biological effects. An overview of the most common phytochemicals is provided, with a particular focus on recent evidence on their antimicrobial activity and its impact on wound treatments, as well as on its antioxidant capacity. 2. In addition to the visual evaluation of antibacterial activity, the colorimetric properties of CPR can be measured by absorbance at λ. or fluorescence excitation at λ, and emission at λ nm for a semi-quantitative value Sicard et al. 2014 Vettiger and Basler, 2016 A. The antibacterial activity was quantified for a total amount of honey using the new method, the phenolic equivalence test and the standard broth microdilution test. These similar values for.