Enzyme Lab Report of Alkaline Phosphatase Biology Essay
Alkaline phosphatase ALP, ALKP 1.3.1 is a hydrolase enzyme responsible for removing phosphate groups from Alkaline phosphatase ALP EC3.I.3.1. is a ubiquitous membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at basic pH. Enzyme kinetics is an essential topic in undergraduate biochemistry courses. A laboratory work covering the most important basic concepts of enzyme kinetics. Alkaline phosphatases ALPs are a group of isoenzymes located on the outer layer of the cell membrane. They catalyze the hydrolysis of organic phosphate esters found in the extracellular space; A very robust and inexpensive kinetic assay is presented for determining the rate of hydrolysis of p-nitrophenyl phosphate by the enzyme alkaline phosphatase. Alkaline phosphatase ALP EC3.I.3.1. is a ubiquitous membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at basic pH values. An in vitro reaction was carried out to investigate the catalytic properties of alkaline phosphatase ALP, a hydrolase enzyme, and the synthetic. A very robust and inexpensive kinetic assay for determining the rates of hydrolysis of p-nitrophenyl phosphate by the enzyme alkaline phosphatase are shown. The reaction rate increases as the pH increases. The enzyme is competitively inhibited by the reaction products, noncompetitively inhibited by L-phenylalanine, and Discussion The data we collected support a correlation between a reduction in alkaline phosphatase activity due to low pH and mutant enzymes. The inhibition of the alkaline phosphatase enzyme by a low blood pH value leads to a reduction in bone density. Mutant AP enzymes in bones contribute to the percentage of laboratory enzyme kinetics of alkaline phosphatase summary. The purpose of this experiment was to measure the maximum velocity Vmax and the dissociation constant Km of the enzyme alkaline phosphatase, and to investigate the effects of two different inhibitors on this enzyme. To do this, the enzyme and the inhibitor,