Determination of reaction rate of enzyme at different temperatures essay
A detailed knowledge and understanding of enzymes, enzymatic reactions and enzyme kinetics is required for a wide range of topics spanning biology, biomedicine and pharmacology. The protocol described above uses lactase as an example of an enzyme relevant to human health for demonstrating enzymatic reactions, provided that: This is known from the experimental observation that the initial rates of enzymes actually decrease at high temperatures, see Fig. an example, and not at all. temperatures the E act E inact equilibration is faster than the time required to initiate the reaction in a stirred spectrophotometer cuvette of the order - and the product line versus time. Furthermore, enzyme reaction rates are affected by several factors, such as temperatures, pH, and even stress in the human body. According to Takai 2004, stress in the human body regulates the release of amylase enzymes and thus determines reaction rates. Conventional understanding of how enzymes function places a strong emphasis on the role of structure. However, mounting evidence clearly indicates that enzymes do not remain fixed or function exclusively in or close to their native structure. Different parts of the enzyme, from individual residues to entire domains, undergo concerted movements at a. The optimal temperature is the balance between the increasing rate of enzyme activity and its decreasing rate due to denaturation. So each enzyme has its optimal temperatures. Conclusion. The rate of the reaction catalyzed by an enzyme is affected by changes in temperature. At temperatures so low,