Green fluorescent protein via heat shock essay
Chalfie et al. published a report in Science showing that the green fluorescent protein GFP from the jellyfish Aequorea victoria could be used as a marker for protein localization. Aggregates of green fluorescent protein GFP-fused truncated N-terminal huntin containing abnormally long polyglutamine channels, of glutamine residue were purified from an ecdysone-inducible mutant neuro2A cell line HD150Q-28 using a fluorescence-activated cell sorter. For analysis, the coding region of the green fluorescent protein GFP from Aequorea victoria was fused to the cauliflower mosaic S promoter and introduced into maize leaf protoplasts. Transient expression of GFP was observed. Furthermore, the coding region of GFP was fused to an Arabidopsis heat shock promoter and co-transformed, Fig. 1: Overview for topology engineering of green fluorescent protein. a Construction of single-domain protein catenane cat-GFP, pseudorotaxane rtx-GFP and cyclic control c-GFP by. Cleaved green fluorescent protein as a tool to study infection with a plant pathogen, cauliflower mosaic virus. PloS one, 14 3, e0213087. Day, RN and Davidson, MW 2009. The fluorescent protein palette: tools for cellular imaging. Chemical Society Reviews, 38 10, 2887-2921. Mechanisms of fluorescent proteins. This collection of articles for the research topic 'Mechanisms of fluorescent proteins' FPs samples a wide range of research on physical mechanisms, applications and molecular engineering strategies. The papers demonstrate a combination of experimental and computational approaches and since the past decade much progress has been made in understanding biological processes involving complex intracellular pathways. The biggest challenge was monitoring and trading metabolites in real time. Although a range of quantitative and imaging techniques have been developed to date, the discovery of green,