Gel Electrophoresis in the Analysis of Sickle Cell Genes Biology Essay
Sickle cell disease SCD is a monogenetic disorder caused by a single base pair point mutation in the β-globin gene, resulting in the replacement of glutamic acid with the amino acid valine in the β-globin chain. Phenotypic variation in the clinical presentation and outcome of the disease is a characteristic feature of the condition. The polymerase chain reaction PCR is a laboratory nucleic acid amplification technique used to denature and renature short segments of deoxyribonucleic acid DNA or ribonucleic acid RNA sequences. Eric Fairfield is a As a private researcher using gel electrophoresis to separate DNA molecules, he won a Ramp D award for the invention of a new method for gel electrophoresis. He answers: DNA. Gel Electrophoresis Gel electrophoresis is a method for separating and analyzing macromolecules such as DNA, RNA and proteins or their fragments, based on their size and charge. In gel electrophoresis, a gel is used as an anti-convective medium and/or sieving medium during electrophoresis. Gels suppress the induced thermal convection. The long fragments of the sickle cell genes will move the slowest compared to the normal genes from which the short fragments were cut. The electrophoresis gel is finally stained and observed under an Ultra Voilet Transilluminator to view the bands produced. This experiment was conducted to outline a scheme for the disease. Sickle cell anemia is one of the most common genetic diseases. This report discusses its pathogenesis and presents classical methods available for its diagnosis. The classical methods identified in this report are hemoglobin electrophoresis, isoelectric focusing and high-performance liquid chromatography. Sickle cell disease SCD is a widespread disease caused by a mutation in the beta globin gene that leads to the production of abnormal hemoglobin called hemoglobin S. The inheritance of the mutation can be homozygous or heterozygous in combination with another hemoglobin mutation. SCD can be characterized by the. For a biology course, G. Allen, Department of Biology, Washington University, discusses electrophoresis in a tutorial on the molecular biology of sickle cell anemia. The issue of The Scientist featured an article by H. Ahern and R. Lewis entitled "Gel Electrophoresis Creates a Revolution." “Sickle Cell Anemia: Spotting a Mutation” is a full-day, research-based biology experience for high school students enrolled in genetics or advanced biology courses. In practice, students use restriction endonuclease digestion, cellulose acetate gel electrophoresis, and microscopy to discover which of the three putative,